Purification and Characterization of the Alanine Aminotransferase from the Hyperthermophilic Archaeon Pyrococcus furiosus and Its Role in Alanine Production

Alanine aminotransferase (AlaAT) was purified from cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by multistep chromatography. The enzyme has an apparent molecular mass of 93.5 kDa, as estimated by gel filtration, and consists of two identical subunits of 46 kDa, as deduced by s...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Ward, Donald E., Kengen, Servé W. M., van der Oost, John, de Vos, Willem M.
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2000
Fag:	Enzymes and Proteins
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC111321/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10762259/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.9.2559-2566.2000
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