Purification and Characterization of the Alanine Aminotransferase from the Hyperthermophilic Archaeon Pyrococcus furiosus and Its Role in Alanine Production

Alanine aminotransferase (AlaAT) was purified from cell extracts of the hyperthermophilic archaeon Pyrococcus furiosus by multistep chromatography. The enzyme has an apparent molecular mass of 93.5 kDa, as estimated by gel filtration, and consists of two identical subunits of 46 kDa, as deduced by s...

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Detalles Bibliográficos

Publicado en:	J Bacteriol
Principais autores:	Ward, Donald E., Kengen, Servé W. M., van der Oost, John, de Vos, Willem M.
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 2000
Assuntos:	Enzymes and Proteins
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC111321/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10762259/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.9.2559-2566.2000
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