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Endogenous oxidative DNA base modifications analysed with repair enzymes and GC/MS technique

GC/MS technique was used to identify endogenous levels of oxidatively modified DNA bases. To avoid possible artefact formation we used Fpg and Endo III endonucleases instead of acid hydrolysis to liberate the base products from unmodified DNA samples. Several different DNA preparations were used: (i...

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Bibliographische Detailangaben
Hauptverfasser: Jaruga, Pawel, Speina, Elzbieta, Gackowski, Daniel, Tudek, Barbara, Olinski, Ryszard
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2000
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC111057/
https://ncbi.nlm.nih.gov/pubmed/10684948
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