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Endogenous oxidative DNA base modifications analysed with repair enzymes and GC/MS technique

GC/MS technique was used to identify endogenous levels of oxidatively modified DNA bases. To avoid possible artefact formation we used Fpg and Endo III endonucleases instead of acid hydrolysis to liberate the base products from unmodified DNA samples. Several different DNA preparations were used: (i...

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Detalhes bibliográficos
Main Authors: Jaruga, Pawel, Speina, Elzbieta, Gackowski, Daniel, Tudek, Barbara, Olinski, Ryszard
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC111057/
https://ncbi.nlm.nih.gov/pubmed/10684948
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