Ligation reaction specificities of an NAD(+)-dependent DNA ligase from the hyperthermophile Aquifex aeolicus
An NAD(+)-dependent DNA ligase from the hyperthermophilic bacterium Aquifex aeolicus was cloned, expressed in Escherichia coli and purified to homogeneity. The enzyme is most active in slightly alkaline pH conditions with either Mg(2+) or Mn(2+) as the metal cofactor. Ca(2+) and Ni(2+) mainly suppor...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2000
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC111035/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10684941/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.6.1447 |
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