Ligation reaction specificities of an NAD(+)-dependent DNA ligase from the hyperthermophile Aquifex aeolicus

An NAD(+)-dependent DNA ligase from the hyperthermophilic bacterium Aquifex aeolicus was cloned, expressed in Escherichia coli and purified to homogeneity. The enzyme is most active in slightly alkaline pH conditions with either Mg(2+) or Mn(2+) as the metal cofactor. Ca(2+) and Ni(2+) mainly suppor...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Tong, Jie, Barany, Francis, Cao, Weiguo
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2000
Fag:	Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC111035/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10684941/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.6.1447
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