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Modified constructs of the tRNA TΨC domain to probe substrate conformational requirements of m(1)A(58) and m(5)U(54) tRNA methyltransferases

The TΨC stem and loop (TSL) of tRNA contains highly conserved nucleoside modifications, m(5)C(49), T(54), Ψ(55) and m(1)A(58). U(54) is methylated to m(5)U (T) by m(5)U(54) methyltransferase (RUMT); A(58) is methylated to m(1)A by m(1)A(58) tRNA methyltransferase (RAMT). RUMT recognizes and methylat...

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Bibliographic Details
Main Authors: Sengupta, Ranjita, Vainauskas, Saulius, Yarian, Connie, Sochacka, Elzbieta, Malkiewicz, Andrzej, Guenther, Richard H., Koshlap, Karl M., Agris, Paul F.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC111031/
https://ncbi.nlm.nih.gov/pubmed/10684932
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