oriT-Directed Cloning of Defined Large Regions from Bacterial Genomes: Identification of the Sinorhizobium meliloti pExo Megaplasmid Replicator Region

We have developed a procedure to directly clone large fragments from the genome of the soil bacterium Sinorhizobium meliloti. Specific regions to be cloned are first flanked by parallel copies of an origin of transfer (oriT) together with a plasmid replication origin capable of replicating large clo...

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Библиографические подробности

Опубликовано в::	J Bacteriol
Главные авторы:	Chain, Patrick S. G., Hernandez-Lucas, Ismael, Golding, Brian, Finan, Turlough M.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 2000
Предметы:	Genetics and Molecular Biology
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10986253/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.19.5486-5494.2000
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