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oriT-Directed Cloning of Defined Large Regions from Bacterial Genomes: Identification of the Sinorhizobium meliloti pExo Megaplasmid Replicator Region

We have developed a procedure to directly clone large fragments from the genome of the soil bacterium Sinorhizobium meliloti. Specific regions to be cloned are first flanked by parallel copies of an origin of transfer (oriT) together with a plasmid replication origin capable of replicating large clo...

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Bibliographische Detailangaben
Hauptverfasser: Chain, Patrick S. G., Hernandez-Lucas, Ismael, Golding, Brian, Finan, Turlough M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2000
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC110993/
https://ncbi.nlm.nih.gov/pubmed/10986253
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