oriT-Directed Cloning of Defined Large Regions from Bacterial Genomes: Identification of the Sinorhizobium meliloti pExo Megaplasmid Replicator Region

We have developed a procedure to directly clone large fragments from the genome of the soil bacterium Sinorhizobium meliloti. Specific regions to be cloned are first flanked by parallel copies of an origin of transfer (oriT) together with a plasmid replication origin capable of replicating large clo...

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Dettagli Bibliografici

Pubblicato in:	J Bacteriol
Autori principali:	Chain, Patrick S. G., Hernandez-Lucas, Ismael, Golding, Brian, Finan, Turlough M.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Genetics and Molecular Biology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10986253/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.19.5486-5494.2000
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