oriT-Directed Cloning of Defined Large Regions from Bacterial Genomes: Identification of the Sinorhizobium meliloti pExo Megaplasmid Replicator Region

We have developed a procedure to directly clone large fragments from the genome of the soil bacterium Sinorhizobium meliloti. Specific regions to be cloned are first flanked by parallel copies of an origin of transfer (oriT) together with a plasmid replication origin capable of replicating large clo...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Chain, Patrick S. G., Hernandez-Lucas, Ismael, Golding, Brian, Finan, Turlough M.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110993/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10986253/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.19.5486-5494.2000
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