Rapid determination of short DNA sequences by the use of MALDI-MS

We have developed a protocol for rapid sequencing of short DNA stretches (15–20 nt) using MALDI-TOF-MS. The protocol is based on the Sanger concept with the modification that double-stranded template DNA is used and all four sequencing reactions are performed in one reaction vial. The sequencing pro...

Full description

Saved in:
Bibliographic Details
Main Authors: Nordhoff, Eckhard, Luebbert, Christine, Thiele, Gabriela, Heiser, Volker, Lehrach, Hans
Format: Artigo
Language:Inglês
Published: Oxford University Press 2000
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC110802/
https://ncbi.nlm.nih.gov/pubmed/11024188
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items