Rapid determination of short DNA sequences by the use of MALDI-MS

We have developed a protocol for rapid sequencing of short DNA stretches (15–20 nt) using MALDI-TOF-MS. The protocol is based on the Sanger concept with the modification that double-stranded template DNA is used and all four sequencing reactions are performed in one reaction vial. The sequencing pro...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Nordhoff, Eckhard, Luebbert, Christine, Thiele, Gabriela, Heiser, Volker, Lehrach, Hans
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2000
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110802/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11024188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.20.e86
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