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Rapid determination of short DNA sequences by the use of MALDI-MS

We have developed a protocol for rapid sequencing of short DNA stretches (15–20 nt) using MALDI-TOF-MS. The protocol is based on the Sanger concept with the modification that double-stranded template DNA is used and all four sequencing reactions are performed in one reaction vial. The sequencing pro...

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Detalhes bibliográficos
Main Authors: Nordhoff, Eckhard, Luebbert, Christine, Thiele, Gabriela, Heiser, Volker, Lehrach, Hans
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC110802/
https://ncbi.nlm.nih.gov/pubmed/11024188
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