Expression of Enzymatically Active Rabbit Hemorrhagic Disease Virus RNA-Dependent RNA Polymerase in Escherichia coli

The rabbit hemorrhagic disease virus (RHDV) (isolate AST/89) RNA-dependent RNA-polymerase (3D(pol)) coding region was expressed in Escherichia coli by using a glutathione S-transferase-based vector, which allowed milligram purification of a homogeneous enzyme with an expected molecular mass of about...

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Main Authors: Vázquez, Ana López, Martín Alonso, José M., Casais, Rosa, Boga, José A., Parra, Francisco
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 1998
主題:
Animal Viruses
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC109747/
https://ncbi.nlm.nih.gov/pubmed/9525622
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