Expression of Enzymatically Active Rabbit Hemorrhagic Disease Virus RNA-Dependent RNA Polymerase in Escherichia coli

The rabbit hemorrhagic disease virus (RHDV) (isolate AST/89) RNA-dependent RNA-polymerase (3D(pol)) coding region was expressed in Escherichia coli by using a glutathione S-transferase-based vector, which allowed milligram purification of a homogeneous enzyme with an expected molecular mass of about...

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Bibliographic Details
Main Authors: Vázquez, Ana López, Martín Alonso, José M., Casais, Rosa, Boga, José A., Parra, Francisco
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1998
Subjects:
Animal Viruses
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC109747/
https://ncbi.nlm.nih.gov/pubmed/9525622
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