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Exploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase α

In order to further understand how DNA polymerases discriminate against incorrect dNTPs, we synthesized two sets of dNTP analogues and tested them as substrates for DNA polymerase α (pol α) and Klenow fragment (exo(−)) of DNA polymerase I (Escherichia coli). One set of analogues was designed to test...

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Detalhes bibliográficos
Main Authors: Kincaid, Kristi, Beckman, Jeff, Zivkovic, Aleksandra, Halcomb, Randall L., Engels, Joachim W., Kuchta, Robert D.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1090547/
https://ncbi.nlm.nih.gov/pubmed/15879351
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki563
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