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Discrimination between Right and Wrong Purine dNTPs by DNA Polymerase I from Bacillus stearothermophilus

We used a series of dATP and dGTP analogues to determine how DNA polymerase I from Bacillus stearothermophilus (BF), a prototypical A family polymerase, uses N-1, N(2), N-3, and N(6) of purine dNTPs to differentiate between right and wrong nucleotide incorporation. Altering any of these nitrogens ha...

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Detalhes bibliográficos
Main Authors: Trostler, Michael, Delier, Alison, Beckman, Jeff, Urban, Milan, Patro, Jennifer N., Spratt, Thomas E., Beese, Lorena S., Kuchta, Robert D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2713353/
https://ncbi.nlm.nih.gov/pubmed/19348507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900104n
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