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Cell-free cotranslation and selection using in vitro virus for high-throughput analysis of protein–protein interactions and complexes
We have developed a simple and totally in vitro selection procedure based on cell-free cotranslation using a highly stable and efficient in vitro virus (IVV). Cell-free cotranslation of tagged bait and prey proteins is advantageous for the formation of protein complexes and allows high-throughput an...
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Cold Spring Harbor Laboratory Press
2005
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1088299/ https://ncbi.nlm.nih.gov/pubmed/15867431 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.3510505 |
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