Cell-free cotranslation and selection using in vitro virus for high-throughput analysis of protein–protein interactions and complexes

We have developed a simple and totally in vitro selection procedure based on cell-free cotranslation using a highly stable and efficient in vitro virus (IVV). Cell-free cotranslation of tagged bait and prey proteins is advantageous for the formation of protein complexes and allows high-throughput an...

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Detalhes bibliográficos
Main Authors: Miyamoto-Sato, Etsuko, Ishizaka, Masamichi, Horisawa, Kenichi, Tateyama, Seiji, Takashima, Hideaki, Fuse, Shinichiro, Sue, Kaori, Hirai, Naoya, Masuoka, Kazuyo, Yanagawa, Hiroshi
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2005
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1088299/
https://ncbi.nlm.nih.gov/pubmed/15867431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.3510505
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