Heterologous Expression of Novobiocin and Clorobiocin Biosynthetic Gene Clusters

A method was developed for the heterologous expression of biosynthetic gene clusters in different Streptomyces strains and for the modification of these clusters by single or multiple gene replacements or gene deletions with unprecedented speed and versatility. λ-Red-mediated homologous recombinatio...

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Autors principals: Eustáquio, Alessandra S., Gust, Bertolt, Galm, Ute, Li, Shu-Ming, Chater, Keith F., Heide, Lutz
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Genetics and Molecular Biology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1087579/
https://ncbi.nlm.nih.gov/pubmed/15870333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.5.2452-2459.2005
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