Point mutation of bacterial artificial chromosomes by ET recombination

Bacterial artificial chromosomes (BACs) offer many advantages for functional studies of large eukaryotic genes. To utilize the potential applications of BACs optimally, new approaches that allow rapid and precise engineering of these large molecules are required. Here, we describe a simple and flexi...

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Main Authors: Muyrers, Joep P.P., Zhang, Youming, Benes, Vladimir, Testa, Giuseppe, Ansorge, Wilhelm, Stewart, A. Francis
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2000
Teme:
Scientific Reports
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1083723/
https://ncbi.nlm.nih.gov/pubmed/11256606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/embo-reports/kvd049
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