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Rapid modification of bacterial artificial chromosomes by ET-recombination.

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The proce...

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Bibliografiske detaljer
Main Authors: Muyrers, J P, Zhang, Y, Testa, G, Stewart, A F
Format: Artigo
Sprog:Inglês
Udgivet: 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148353/
https://ncbi.nlm.nih.gov/pubmed/10037821
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