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Gene Cloning and Characterization of Recombinant RNase HII from a Hyperthermophilic Archaeon

We have cloned the gene encoding RNase HII (RNase HII(Pk)) from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 by screening of a library for clones that suppressed the temperature-sensitive growth phenotype of an rnh mutant strain of Escherichia coli. This gene was expressed in an rnh...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Haruki, Mitsuru, Hayashi, Keiko, Kochi, Takayuki, Muroya, Ayumu, Koga, Yuichi, Morikawa, Masaaki, Imanaka, Tadayuki, Kanaya, Shigenori
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107705/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9829929/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.23.6207-6214.1998
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