Gene Cloning and Characterization of Recombinant RNase HII from a Hyperthermophilic Archaeon

We have cloned the gene encoding RNase HII (RNase HII(Pk)) from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 by screening of a library for clones that suppressed the temperature-sensitive growth phenotype of an rnh mutant strain of Escherichia coli. This gene was expressed in an rnh...

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Pubblicato in:	J Bacteriol
Autori principali:	Haruki, Mitsuru, Hayashi, Keiko, Kochi, Takayuki, Muroya, Ayumu, Koga, Yuichi, Morikawa, Masaaki, Imanaka, Tadayuki, Kanaya, Shigenori
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1998
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107705/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9829929/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.23.6207-6214.1998
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