Gene Cloning and Characterization of Recombinant RNase HII from a Hyperthermophilic Archaeon

We have cloned the gene encoding RNase HII (RNase HII(Pk)) from the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 by screening of a library for clones that suppressed the temperature-sensitive growth phenotype of an rnh mutant strain of Escherichia coli. This gene was expressed in an rnh...

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Hauptverfasser: Haruki, Mitsuru, Hayashi, Keiko, Kochi, Takayuki, Muroya, Ayumu, Koga, Yuichi, Morikawa, Masaaki, Imanaka, Tadayuki, Kanaya, Shigenori
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1998
Schlagworte:
Enzymes and Proteins
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC107705/
https://ncbi.nlm.nih.gov/pubmed/9829929
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