Single-Amino-Acid Substitutions in Open Reading Frame (ORF) 1b-nsp14 and ORF 2a Proteins of the Coronavirus Mouse Hepatitis Virus Are Attenuating in Mice

A reverse genetic system was recently established for the coronavirus mouse hepatitis virus strain A59 (MHV-A59), in which cDNA fragments of the RNA genome are assembled in vitro into a full-length genome cDNA, followed by electroporation of in vitro-transcribed genome RNA into cells with recovery o...

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Autors principals: Sperry, Steven M., Kazi, Lubna, Graham, Rachel L., Baric, Ralph S., Weiss, Susan R., Denison, Mark R.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Pathogenesis and Immunity
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1075728/
https://ncbi.nlm.nih.gov/pubmed/15731233
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.79.6.3391-3400.2005
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