Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli
Replacement of Escherichia coli’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | J Bacteriol |
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| Κύριος συγγραφέας: | |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
American Society for Microbiology (ASM)
1998
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107131/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9555887/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.8.2063-2071.1998 |
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