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Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli
Replacement of Escherichia coli’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology
1998
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC107131/ https://ncbi.nlm.nih.gov/pubmed/9555887 |
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