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Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

Replacement of Escherichia coli’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is...

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Autor principal: Murphy, Kenan C.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1998
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC107131/
https://ncbi.nlm.nih.gov/pubmed/9555887
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