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Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli

Replacement of Escherichia coli’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Autor principal: Murphy, Kenan C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107131/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9555887/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.8.2063-2071.1998
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