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Biosynthesis of Mannose-Containing Lipid-Linked Oligosaccharides by Solubilized Enzyme Preparation from Cultured Soybean Cells

Glycosyl transferases that participate in the assembly of the lipid-linked oligosaccharide intermediates were solubilized from cultured soybean cells using 0.3% Nonidet P-40 (NP-40) in the presence of 10% glycerol. The solubilized enzyme preparation was reasonably stable and 50% of the activity stil...

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Detalhes bibliográficos
Main Authors: Hori, Hidetaka, Kaushal, Gur P., Elbein, Alan D.
Formato: Artigo
Idioma:Inglês
Publicado em: 1985
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1064616/
https://ncbi.nlm.nih.gov/pubmed/16664147
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