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Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes

A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon’s site of insertion has been developed. The basic plasposon, named TnMod, consists of the Tn5 inverted repeats, a conditional origin of replication, rare restriction endo...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Dennis, Jonathan J., Zylstra, Gerben J.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1998
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106450/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9647854/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.7.2710-2715.1998
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