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Plasposons: Modular Self-Cloning Minitransposon Derivatives for Rapid Genetic Analysis of Gram-Negative Bacterial Genomes

A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon’s site of insertion has been developed. The basic plasposon, named TnMod, consists of the Tn5 inverted repeats, a conditional origin of replication, rare restriction endo...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Dennis, Jonathan J., Zylstra, Gerben J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106450/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9647854/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.7.2710-2715.1998
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