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Direct Detection of Viable Bacteria, Molds, and Yeasts by Reverse Transcriptase PCR in Contaminated Milk Samples after Heat Treatment

A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1α)-specific primers, the detection level was 10 cells ml of milk(−1). The simplicity and...

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Hlavní autoři: Vaitilingom, Marc, Gendre, Francois, Brignon, Pierre
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 1998
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC106384/
https://ncbi.nlm.nih.gov/pubmed/9501455
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