Direct Detection of Viable Bacteria, Molds, and Yeasts by Reverse Transcriptase PCR in Contaminated Milk Samples after Heat Treatment

A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1α)-specific primers, the detection level was 10 cells ml of milk(−1). The simplicity and...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Vaitilingom, Marc, Gendre, Francois, Brignon, Pierre
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1998
Soggetti:	Food Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106384/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9501455/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.3.1157-1160.1998
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