Chaperone Coexpression Plasmids: Differential and Synergistic Roles of DnaK-DnaJ-GrpE and GroEL-GroES in Assisting Folding of an Allergen of Japanese Cedar Pollen, Cryj2, in Escherichia coli

Plasmids that can be used for controlled expression of the DnaK-DnaJ-GrpE and/or GroEL-GroES chaperone team were constructed in order to facilitate assessment of the effects of these chaperone teams on folding or assembly of recombinant proteins in Escherichia coli. A typical pACYC184-based plasmid...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Nishihara, Kazuyo, Kanemori, Masaaki, Kitagawa, Masanari, Yanagi, Hideki, Yura, Takashi
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1998
Sujets:	Enzymology and Protein Engineering
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106217/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9572938/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.5.1694-1699.1998
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