Chaperone Coexpression Plasmids: Differential and Synergistic Roles of DnaK-DnaJ-GrpE and GroEL-GroES in Assisting Folding of an Allergen of Japanese Cedar Pollen, Cryj2, in Escherichia coli

Plasmids that can be used for controlled expression of the DnaK-DnaJ-GrpE and/or GroEL-GroES chaperone team were constructed in order to facilitate assessment of the effects of these chaperone teams on folding or assembly of recombinant proteins in Escherichia coli. A typical pACYC184-based plasmid...

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Bibliographic Details
Main Authors: Nishihara, Kazuyo, Kanemori, Masaaki, Kitagawa, Masanari, Yanagi, Hideki, Yura, Takashi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1998
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC106217/
https://ncbi.nlm.nih.gov/pubmed/9572938
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