Chaperone Coexpression Plasmids: Differential and Synergistic Roles of DnaK-DnaJ-GrpE and GroEL-GroES in Assisting Folding of an Allergen of Japanese Cedar Pollen, Cryj2, in Escherichia coli
Plasmids that can be used for controlled expression of the DnaK-DnaJ-GrpE and/or GroEL-GroES chaperone team were constructed in order to facilitate assessment of the effects of these chaperone teams on folding or assembly of recombinant proteins in Escherichia coli. A typical pACYC184-based plasmid...
সংরক্ষণ করুন:
| প্রকাশিত: | Appl Environ Microbiol |
|---|---|
| প্রধান লেখক: | , , , , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
American Society for Microbiology (ASM)
1998
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106217/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9572938/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.5.1694-1699.1998 |
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