Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for th...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Fitzgerald, Nancy B., English, R. Samuel, Lampel, Jay S., Vanden Boom, Thomas J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106195/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9546194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.4.1580-1583.1998
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