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Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for th...

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Hlavní autoři: Fitzgerald, Nancy B., English, R. Samuel, Lampel, Jay S., Vanden Boom, Thomas J.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 1998
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC106195/
https://ncbi.nlm.nih.gov/pubmed/9546194
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