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Sonication-Dependent Electroporation of the Erythromycin-Producing Bacterium Saccharopolyspora erythraea

We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for th...

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Detalles Bibliográficos
Main Authors: Fitzgerald, Nancy B., English, R. Samuel, Lampel, Jay S., Vanden Boom, Thomas J.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1998
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC106195/
https://ncbi.nlm.nih.gov/pubmed/9546194
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