Engineering of a Cold-Adapted Protease by Sequential Random Mutagenesis and a Screening System

A cold-adapted protease subtilisin was successfully isolated by evolutionary engineering based on sequential in vitro random mutagenesis and an improved method of screening (H. Kano, S. Taguchi, and H. Momose, Appl. Microbiol. Biotechnol. 47:46–51, 1997). The mutant subtilisin, termed m-63, exhibite...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Taguchi, Seiichi, Ozaki, Akiyoshi, Momose, Haruo
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1998
Subjects:	Enzymology and Protein Engineering
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106071/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464383/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.492-495.1998
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