Engineering of a Cold-Adapted Protease by Sequential Random Mutagenesis and a Screening System

A cold-adapted protease subtilisin was successfully isolated by evolutionary engineering based on sequential in vitro random mutagenesis and an improved method of screening (H. Kano, S. Taguchi, and H. Momose, Appl. Microbiol. Biotechnol. 47:46–51, 1997). The mutant subtilisin, termed m-63, exhibite...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Taguchi, Seiichi, Ozaki, Akiyoshi, Momose, Haruo
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106071/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9464383/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.2.492-495.1998
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