Properties of Mutant SHV-5 β-Lactamases Constructed by Substitution of Isoleucine or Valine for Methionine at Position 69

The effect of replacement of Met-69 by Ile or Val on the properties of the extended-spectrum β-lactamase SHV-5 was studied. Mutant enzymes were constructed by site-specific mutagenesis and expressed under isogenic conditions in Escherichia coli DH5α cells. Compared with SHV-5, the mutant β-lactamase...

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Detalhes bibliográficos

Publicado no:	Antimicrob Agents Chemother
Principais autores:	Giakkoupi, Panagiota, Miriagou, Vivi, Gazouli, Maria, Tzelepi, Eva, Legakis, Nicholas J., Tzouvelekis, Leonidas S.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Mechanisms of Resistance
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105805/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9593168/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.42.5.1281
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