Properties of Mutant SHV-5 β-Lactamases Constructed by Substitution of Isoleucine or Valine for Methionine at Position 69
The effect of replacement of Met-69 by Ile or Val on the properties of the extended-spectrum β-lactamase SHV-5 was studied. Mutant enzymes were constructed by site-specific mutagenesis and expressed under isogenic conditions in Escherichia coli DH5α cells. Compared with SHV-5, the mutant β-lactamase...
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| Опубликовано в:: | Antimicrob Agents Chemother |
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| Главные авторы: | , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1998
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105805/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9593168/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.42.5.1281 |
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