Properties of Mutant SHV-5 β-Lactamases Constructed by Substitution of Isoleucine or Valine for Methionine at Position 69

The effect of replacement of Met-69 by Ile or Val on the properties of the extended-spectrum β-lactamase SHV-5 was studied. Mutant enzymes were constructed by site-specific mutagenesis and expressed under isogenic conditions in Escherichia coli DH5α cells. Compared with SHV-5, the mutant β-lactamase...

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Veröffentlicht in:	Antimicrob Agents Chemother
Hauptverfasser:	Giakkoupi, Panagiota, Miriagou, Vivi, Gazouli, Maria, Tzelepi, Eva, Legakis, Nicholas J., Tzouvelekis, Leonidas S.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1998
Schlagworte:	Mechanisms of Resistance
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105805/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9593168/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.42.5.1281
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