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Selection and Characterization of β-Lactam–β-Lactamase Inactivator-Resistant Mutants following PCR Mutagenesis of the TEM-1 β-Lactamase Gene

Mechanism-based inactivators of β-lactamases are used to overcome the resistance of clinical pathogens to β-lactam antibiotics. This strategy can itself be overcome by mutations of the β-lactamase that compromise the effectiveness of their inactivation. We used PCR mutagenesis of the TEM-1 β-lactama...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Vakulenko, Sergei B., Geryk, Bruce, Kotra, Lakshmi P., Mobashery, Shahriar, Lerner, Stephen A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1998
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC105642/
https://ncbi.nlm.nih.gov/pubmed/9660980
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