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Improved Amplification of Microbial DNA from Blood Cultures by Removal of the PCR Inhibitor Sodium Polyanetholesulfonate

Molecular methods are increasingly used to identify microbes in clinical samples. A common technical problem with PCR is failed amplification due to the presence of PCR inhibitors. Initial attempts at amplification of the bacterial 16S rRNA gene from inoculated blood culture media failed for this re...

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Detalhes bibliográficos
Main Authors: Fredricks, David N., Relman, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC105069/
https://ncbi.nlm.nih.gov/pubmed/9738025
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