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Amplification of Guthrie card DNA: Effect of guanidine thiocyanate on binding of natural whole blood PCR inhibitors

Amplification of DNA from whole blood collected on Guthrie card filter paper presents considerable technical obstacles due to the presence of natural PCR inhibitors (protein, heavy metals, heme, and heme degradation products) and low copy number of genomic material. For this purpose we evaluated gua...

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Detalhes bibliográficos
Publicado no:J Clin Lab Anal
Main Authors: Makowski, Gregory S., Davis, Esther L., Hopfer, Sidney M.
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6760716/
https://ncbi.nlm.nih.gov/pubmed/9058242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/(SICI)1098-2825(1997)11:2<87::AID-JCLA4>3.0.CO;2-H
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