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Amplification of Guthrie card DNA: Effect of guanidine thiocyanate on binding of natural whole blood PCR inhibitors
Amplification of DNA from whole blood collected on Guthrie card filter paper presents considerable technical obstacles due to the presence of natural PCR inhibitors (protein, heavy metals, heme, and heme degradation products) and low copy number of genomic material. For this purpose we evaluated gua...
Gespeichert in:
| Veröffentlicht in: | J Clin Lab Anal |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Wiley Subscription Services, Inc., A Wiley Company
1998
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6760716/ https://ncbi.nlm.nih.gov/pubmed/9058242 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/(SICI)1098-2825(1997)11:2<87::AID-JCLA4>3.0.CO;2-H |
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