Use of Lambda Phage DNA as a Hybrid Internal Control in a PCR-Enzyme Immunoassay To Detect Chlamydia pneumoniae

An inherent problem in the diagnostic PCR assay is the presence of ill-defined inhibitors of amplification which may cause false-negative results. Addition of an amplifiable fragment of foreign DNA in the PCR to serve as a hybrid internal control (HIC) would allow for a simple way to identify specim...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Pham, Dien G., Madico, Guillermo E., Quinn, Thomas C., Enzler, Mark J., Smith, Thomas F., Gaydos, Charlotte A.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1998
Sujets:	Chlamydiology and Rickettsiology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104952/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9650936/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.7.1919-1922.1998
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