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Use of Lambda Phage DNA as a Hybrid Internal Control in a PCR-Enzyme Immunoassay To Detect Chlamydia pneumoniae

An inherent problem in the diagnostic PCR assay is the presence of ill-defined inhibitors of amplification which may cause false-negative results. Addition of an amplifiable fragment of foreign DNA in the PCR to serve as a hybrid internal control (HIC) would allow for a simple way to identify specim...

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Autori principali: Pham, Dien G., Madico, Guillermo E., Quinn, Thomas C., Enzler, Mark J., Smith, Thomas F., Gaydos, Charlotte A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC104952/
https://ncbi.nlm.nih.gov/pubmed/9650936
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